Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A–F) Static time kill experiments represent the change in the viable count of M. tuberculosis (closed squares) and M. komossense (open circles) in response to exposure to isoniazid (H) at the displayed multiple of MIC [1 mg/L (concentrations used-0.25–8 mg/L)].

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques:

Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A–F) Static time kill experiments represent the change in the viable count of M. tuberculosis (closed squares) and M. komossense (open circles) in response to exposure to rifampicin (R) at the displayed multiple of MIC [0.4 mg/L (concentrations used- 0.1-3.2 mg/L)].

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques:

Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A,B) Change in the viable count of M. komossense in the hollow fiber system in response to rifampicin alone or in combination with isoniazid at the displayed multiples of MIC. Data displayed are mean values of three technical replicates.

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques:

Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A) Nile red staining of M. komossense of bacteria harvested from a 168 h hollow fiber experiment where bacteria were exposed to isoniazid at 1 × MIC (1 mg/L) Green flourescence indicates the presence of one or more lipid bodies in the bacterial cells. Red fluorescence is the result of the stain interacting with the polar lipids in the bacterial outer envelope. Individual samples of bacteria were defined as S= susceptibile or R= resistant based on a microbroth dilution suceptibility test conducted agaisnt isoniazid, rifampicin, ethambutol, pretomanid, and bedaquiline. (B) Lipid body staining of M. tuberculosis was achieved with lipophillic Nile red. Green flourescence indicates the presence of one or more lipid bodies in the bacterial cells. Red fluorescence is the result of the stain interacting with the polar lipids in the bacterial outer envelope. Individual samples of bacteria were defined as S = susceptibile or R = resistant based on a microbroth dilution suceptibility test conducted agaisnt isoniazid, rifampicin, ethambutol, pretomanid, and bedaquiline.

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques: Staining, Bacteria, Fluorescence